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BLOOD COLLECTION
FASTING MEANS SAMPLE TO COLLECT AFTER 10- 12 HRS OF MEAL. NOTHING SHOULD BE TAKEN IN BETWEEN EXCEPT WATER Blood composition is significantly altered after consuming food, and consequently alters many clinical chemistry tests. If a patient has eaten, and the physician still wants the test, then "non-fasting" is written on the request so the laboratory can make a notation on the report as to why some of the test values may be different than expected Following samples should be collected in morning between- 7 :00 –9 :00 AM
Three samples at ½ hourly interval are required for- LH/FSH/Prolactin( pooled sera is required for examination) Amount of blood to be taken : Before collecting the sample one has to go through the directory of service to check the amount of blood required for various tests. However general guide line is— As Evacuated tube systems are in use – transfer required amount as mentioned in each tube For up to 10 biochem. Tests min amount.......... 3.0 ml If Serology/ hormonal tests also ..........................4.0 ml For Iron Profile....................................... ...2.0 ml blood extra FOR IONIC CALCIUM AND TOTAL CALCIUM ESTIMATION PREFER SAMPLE COLLECTION WITH OUT TORNIQUET APPLICATION. If not possible reduce tourniquet application time to 1 mint For immunology/ hormone, electrolytes including Calcium assays samples should be collected preferably in Gel tube/SST. Unless specified.
The vaccutainer system colour codes :
SNo.
Colour of the cap
Anticoagulant
Used for
1
Red
None
Test Requiring Sera ( Urea/ Cret/ Na/K /Ca/Mg, LFT/ Hormones/ Lipid, GGT, CK, Trop-T, Prot/ Alb , Homocystine
2
Blue
Sodium Citric Acid
Coagulation Studies [ PT?PC?INR, APTT< FDP/ D-Dimer / Fibrinogen
3
Green
Sodium Heparin
Flow Cytometry/ Cytogenetics
( HLS B-27, CD-4/8)
4
Lavender
EDTA
CBc, , G6 PD, Malaria/ Filaria,, Hb Electrophoresis
5
Grey
Sodium Fluoride & Potassium Oxalate
Glucose
6
Black
Sodium Citrate
W G ESR
7
Yellow top with gel
S.electrolytes, S.Calcium, and tests where it is essential to separate serum from RBCs earliest.
Amount of blood to be taken : Before collecting the sample one has to go through the directory of service to check the amount of blood required for various tests. However general guide line is— As Evacuated tube systems are in use – transfer required amount as mentioned in each tube For up to 10 biochem. Tests min amount.......... 3.0 ml If Serology/ hormonal tests also ..........................4.0 ml For Iron Profile....................................... ...2.0 ml blood extra FOR IONIC CALCIUM AND TOTAL CALCIUM ESTIMATION PREFER SAMPLE COLLECTION WITH OUT TORNIQUET APPLICATION. If not possible reduce tourniquet application time to 1 mint For immunology/ hormone, electrolytes including Calcium assays samples should be collected preferably in Gel tube/SST. Unless specified.
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