Blood Culture Sample Collection

Blood Culture Sample Collection:-

1. Procedure for Blood culture by Automated Culture system.-
Sample can be sent in BacT/ALERT FA bottle (for adult) or pedi BacT bottle (for pediatric patient`s). These are provided by the Department after receiving requisition forms with complete clinical details of the patient, signed by consulting clinician .

2. Correct specimen collection is extremely important when obtaining blood culture

• Disinfect the patients venipuncture site with 70% alcohol swab or commonly available alcohol pad and then with 1-2% tincture iodine.
• Cleanse the site in circular motion from the center to periphery.
• Allow the area to dry to prevent hemolysis of the specimen.

3. Procedure Of Blood Collection

• Remove the plastic flip top from culture bottle and disinfect with alcohol swab.
• Blood culture bottles must be at room temperatureand Do not write anything on the bottle. write the barcode number of the bottle on the patient,s form.
• Draw 10 ml/bottle of patient`s sample (for adult) and 4ml/bottle (for pediatric age group) and transfer it into culture bottle using aseptic technique.
• If using Needle and syringe method for Blood collection Transfer Blood in to ANAEROBIC BOTTLE FIRST then AEROBIC BOTTLE.equally (specially in case suspected SEPSIS.
• Transport the inoculated bottle immediately to the laboratory. DO NOT REFRIGERATE THE BOTTLE AT ANY STAGE.
• TIMINGS: Chowk—24 hrs except on last day of every month.
• Kapoorthala—8:00 AM to 9:00PM ONLY .

IMPORTANT PRECAUTIONS: